Figure 6. The anti-inflammatory effect of OTC in cultured RPE occurs independent of SLC5A8 expression. Mouse primary retinal pigment
epithelial (mRPE) cells were prepared from Slc5a8+/+ (wild-type, WT) and Slc5a8-/- mouse retinas and exposed to tumor necrosis factor-α (TNF-α; 10 ng/mL; 24 h incubation) in the presence or absence of L-2-oxothiazolidine-4-carboxylic
acid (OTC; 0.5 mM) and nicotinic acid (NA; 1 mM; positive control). Quantitative polymerase chain reaction (qPCR) analysis
of (A) interleukin-6 (IL-6) and (B) chemokine (C-C) motif ligand 2 (Ccl2) mRNA expression. Cell culture medium was then collected and used for enzyme-linked
immunosorbent assay (ELISA) analysis of (C) IL-6 protein. *p<0.01 compared with control cells; **p<0.01 compared to TNF-α-treated cells.