Figure 4. OTC attenuates the expression of IL-1β and TGF-β, and enhances expression of ABCA1 and ABCG1 in the Ccl2^−/−/Cx3cr1^−/− double knockout mouse retina. Enzyme-linked immunosorbent assay (ELISA) analysis of (A) interleukin-1β (IL-1β) and (B) transforming growth factor-β (TGF-β) protein; western blot analysis of (C) adenosine triphosphate-binding cassette sub-family G member 1 (ABCG1) and adenosine triphosphate-binding cassette sub-family A member 1 (ABCA1) proteins in the neural retina (NR) and retinal pigment epithelial (RPE) and eyecup samples were obtained from double knockout (DKO rd8) mice treated with L-2-oxothiazolidine-4-carboxylic acid (OTC DKO rd8) or regular drinking water (Con DKO rd8) for a period of 5 months. *p<0.05 compared to corresponding control; **p<0.01 compared to corresponding control.