Table 2 of
Singh, Mol Vis 2014; 20:704-713. Table 2 of
Singh, Mol Vis 2014; 20:704-713.
Table 2. PCR conditions for TLR4 SNPs genotyping.
| SNP ID | Primers (5’-3’) | Restriction enzyme used | Product size and genotypes |
|---|---|---|---|
| rs4986790 | F: CTGCTCTAGAGGGCCTGTG | BccI | 140=AA 140, 77, 63=AG 77, 63=GG |
| R: TTCAATAGTCACACTCACCAG | |||
| rs4986791 | F: CTACCAAGCCTTGAGTTTCTG | BslI | 110=TT 110, 89, 22=TC 89, 22=CC |
| R: AAGCTCAGATCTAAATACT | |||
| rs10759931 | F: ATAACCTCAGTGGGCTCTGG | KpnI | 241=AA 241, 190, 51=AG |
| R: ATGTTCTGGCATCTGGGAAG | |||
| rs1927911 | F: TCACTTTGCTCAAGGGTCAA | StyI | 203=TT 203, 178, 25=TC 178, 25=CC |
| R: AAACCTGCATGCTCTGCAC | |||
| rs1927914 | F: ACAAAATGGTCCCTCACAGC | SphI | 150=TT 157, 90, 67=TC 90, 67=CC |
| R: TGGAAAGTAGCAAGTGCAATG |