Figure 1. Gels showing PCR-RFLP analysis of different SNPs of the TLR4 gene. The amplified products of the SNPs rs4986790, rs4986791, rs10759931, rs1927911, and rs1927914 were digested with restriction enzymes BccI, BslI, KpnI, StyI and SphI, respectively. The restricted products were separated on 3% agarose gel, according to our previous report [10]. A: For genotyping rs4986790, the 140-bp PCR product was digested with BccI. The A allele is not cut by the enzyme, whereas the G allele yields 77 and 63 bp products. B: For genotyping rs4986791, the 110-bp PCR product was digested with BslI. The T allele is not cut by the enzyme, whereas the C allele yields 89 and 21 bp products. C: For genotyping rs1927911, the 203-bp PCR product was digested with StyI. The T allele is not cut by the enzyme, whereas the C allele yields 178 and 25 bp products. D: For genotyping rs10759931, the 241-bp PCR product was digested with KpnI. The A allele is not cut by the enzyme, whereas the G allele yields 190 and 51 bp products. E: For genotyping rs1927914, the 157-bp PCR product was digested with SphI. The T allele is not cut by the enzyme, whereas the C allele yields 90 and 67 bp products.