Table 1 of
Guo, Mol Vis 2014; 20:670-682. Table 1 of
Guo, Mol Vis 2014; 20:670-682.
Table 1. List of primary antibodies used in this study.
| Antibody | Production | Host | Working dilution | Company | Cat No. | Immunogen | Reference |
|---|---|---|---|---|---|---|---|
| Anti-Cx43 | Polyclonal | Rb | 1:1000 | Sigma-Aldrich, | C6219 | Synthetic peptide corresponding to amino acids 363–382 of human and rat Cx43 | [9] |
| Anti-Cx36 | Polyclonal | Rb | 1:400 | Life Technologies, | 51–6300 | Peptide corresponding to a sequence from the C-terminus of rat and mouse Cx36 | [54] |
| Anti-Cx45 | Polyclonal | Rb | 1:100 | Life Technologies, | 40–7000 | Synthetic peptide derived from the C-terminal region of the mouse, rat, Chinese hamster, and golden hamster Cx45 | [55] |
| Anti-CD45 | Monoclonal, clone OX-1 | Ms | 1:20 | BD PharMingen | 550,566 | CD45-enriched glycoprotein fraction from Wistar rat thymocytes | [56] |
| Anti-nitrotyrosine | Monoclonal, clone 1A6 | Ms | 1:2000 | Millipore Corporation, MA | 05–233 | Nitrated KLH (Keyhole Limpet Hemocyanin) | [57,58] |
| Anti-Iba1 | Polyclonal | Gt | 1:250 | Abcam | Ab5076 | Synthetic peptide corresponding to amino acids 135–147 of human Iba1 | [59,60] |