Figure 1. An overview, hematoxylin and eosin (H&E) staining, and immunohistochemical staining of malignant melanoma. A: An overview of the tumor. B: H&E staining of the tissue of malignant melanoma. C: H&E staining depigmented by KMnO₄ (potassium permanganate) of malignant melanoma. The tissue was constituted by fusiform and epithelioid malignant melanoma cells. Cytoplasm was abundant with melanin granules. Nuclei was big with prominent nucleoli. D: Expression of HMB-45 in immunohistochemical staining of malignant melanoma was positive. E: Expression of Melan-A was weakly positive; F: Expression of S-100 protein was positive. G: Negative control. DAB was used as chromogen. (magnification: 200×).