Figure 4. Analysis of cell migration after gene silencing (n = 4), using a modified Boyden chamber. Cell migration is shown with arrows. A: Migration of normal corneal fibroblast. B: Migration of cells after treatment with scrambled siRNA. C: Migration of cells after treatment of corneal fibroblast with GAPDH siRNA. D: Migration of cells after treatment of normal corneal fibroblast with human antigen R (HuR) siRNA. E: Migration of cells after treatment of normal corneal fibroblast with β-actin siRNA. F: Bar graph showing cell migration after different treatments (A to E). Cells were counted from six different frames. All the treatments were performed in tetraplicate, and standard deviations were calculated.