Figure 7 of Gnana-Prakasam, Mol Vis 2014; 20:561-574.

Figure 7. Bone morphogenetic protein signaling in wild-type and Tmprss6msk/msk mouse retinas. A: Retinal cryosections from wild-type (WT) and Tmprss6msk/msk (KO) mice were subjected to immunofluorescence to detect Smad5 and phosphorylated Smad1, Smad5, and Smad8 (pSmad1/5/8). The experiment was repeated with two mice (four retinas) for each genotype. B: qPCR analysis was performed to quantify ID1 (inhibitor of differentiation-1) mRNA levels in 3-month-old and 5-month-old wild-type (WT) and Tmprss6msk/msk (KO) mouse retinas. Data are presented as the fold change in the KO retina relative to the WT retina. Data represent the results from three independent RNA preparations (three wild-type mice and three Tmprss6msk/msk mice). ***, p<0.001.