Figure 6. Expression of hemojuvelin and hepcidin in wild-type and Tmprss6^msk/msk mouse retinas. A: Immunofluorescent staining of retinal cryosections from 4-week-old wild-type (WT) and Tmprss6^msk/msk (KO) mice was performed for hemojuvelin and hepcidin. The experiment was repeated with two mice (four retinas) for each genotype. B: The neural retina and the RPE/ eyecup were isolated from the wild-type and Tmprss6^msk/msk mice, and the RNA samples isolated from these preparations were used for qPCR to assess hepcidin mRNA levels. Data represent the results from three independent RNA preparations (three wild-type mice and three Tmprss6^msk/msk mice). *** p<0.001.