Figure 4. Expression of H-ferritin and L-ferritin and reverse transcription-polymerase chain reaction (RT-PCR)/quantitative PCR (qPCR) analysis of mRNA levels for various iron-regulatory genes in wild-type and Tmprss6^msk/msk mouse retinas. A: Expression of H-ferritin and L-ferritin was examined with immunofluorescence in 4-week-old wild-type and Tmprss6^msk/msk mouse retinal cryosections. Results are representative of the experiments performed with two wild-type mice and two Tmprss6^msk/msk mice; two different retinal sections were examined for each mouse. B: RT–PCR and qPCR analyses of mRNA levels of various iron-regulatory genes in wild-type and Tmprss6^msk/msk mouse retinas (RPE/eyecup plus neural retina). 18S was used as an internal control. The values (mean±SE), obtained with RNA preparations from the retinas of three wild-type mice and three Tmprss6^msk/msk mice, represent fold changes in mRNA levels in the knockout mouse retina compared to the wild-type retina (KO/WT) as assessed with qPCR.