Table 2 of Furgoch, Mol Vis 2014; 20:535-544.

Table 2. CHM cDNA primers.

Direction CHM RT–PCR cDNA amplicon Start bp Tm (°C)
FWD TAATAGTCACATGACACGTTTCCC 1 54.8
REV TTTAAAATGAGCAAGTCAATGTGC 2200 52.8
Direction CHM cDNA sequencing Start bp Tm (°C)
FWD CGGAGTTTGATGTGATCGTAATAG 100 53.4
FWD CAGCGATCCAGAGAATGC 560 53.4
FWD TACCAGGATTCTTGCATTTCG 860 52.9
FWD TTTGGTGGAATTTATTGTCTTCG 1260 51.7
FWD ATTTGACTTGCACATCTTCTAAAACA 1600 53.7
REV AGGCAGGAAGGCAGAATCT 400 56.2
REV CCTGTTCCACTCGTCCTTCT 800 56.4
REV AGGCACTGTACTGAATGGCG 1200 57.6
REV TTGTTCATTTTCTATCTCCATTTCAG 1600 51.9
REV TAGACTCTGAGACCAGTCAGAATTTC 2000 55.6

RT–PCR and cDNA sequencing primers for choroideremia open reading frame

Furgoch, Mol Vis 2014; 20:535-544. http://www.molvis.org/molvis/v20/535

©2014 Molecular Vision http://www.molvis.org/molvis/

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