Molecular Vision: Furgoch, Mol Vis 2014; 20:535-544. Table 1

Table 1. Details of the primer pairs used to amplify the exons of the CHM gene.

Exon		Direction	Primer	Amplicon (bp)	Tm (°C)
1	*	FWD	GACCTTCCACCCAAGAACTAC	216	55.0
		REV	ACAGTCTTCCTAAACTTTGTCC		52.4
2	+	FWD	CAGCAATGGCATGTATTGAACATT	384	54.6
		REV	TGATGCATTTGGTTTATTCTCAAAGA		53.6
3	Δ	FWD	AATTGTTACAGAAGAAGCTACTATGG	160	52.9
		REV	GGTTTTCTTCAGTGCAGGGTTA		55.3
4	Δ	FWD	TTTTTCTCCCCTTCCTTTGA	411	51.5
		REV	TGTAGCAACCAATGCCACAT		55.1
5	Δ	FWD	TTCCAATACAGTTCCCCTGT	648	53.4
		REV	TGTTTTCAATGCAAAGATGG		49.4
6	Δ	FWD	ATGGATCAGGTTTTGCTGCT	481	54.9
		REV	ACCACGGAGGACTGGAATTT		56.3
7	Δ	FWD	GGGAAAAAAGTGTAATTTGG	330	47.4
		REV	TGGCTAACCTATTGATAGTGC		52.0
8	Δ	FWD	TGTCCAAAATGTAATACCACC	402	50.5
		REV	TCAAGTATCACTTTTAGAAGGG		49.7
9	Δ	FWD	TCTGGTTTGCTCACAGTTCT	346	54.0
		REV	TGAAGGTTACTTATATCATCCTTAC		49.9
10	Δ	FWD	GCCCTCAAAATAGCAACAAGA	452	53.4
		REV	GGCTTCCCTAAAACCAGACC		55.6
11	Δ	FWD	GGGAGGTGACACTTTTATCC	500	52.8
		REV	AGAGAGTAAAACGGTGCTTG		52.6
12	Δ	FWD	AAATATGTTTCAAATTCTGTTCCAAA	436	50.6
		REV	AAGGGGATGGTGTGAAATGA		54.0
13	*	FWD	CGCTCAGCTCTCTATTATCCAT	257	53.8
		REV	CCGGAAGATTATGATGGTTACAT		52.5
14	*	FWD	TAGGCTACACAGTGTAGTAA	322	49.6
		REV	GACTTCTCTCCTCCCAGAGG		56.1
15	Δ	FWD	TGAGGTACTGCCATCCTTGA	478	53.9
		REV	TGAGACCAGTCAGAATTTCCAA		55.5