Figure 4. The RT-PCR result with primers specific to the coding region of the CHM mRNA showing a 2.2 kb amplicon. Size marker (“M”): GeneRuler 1kb+ DNA Ladder (Thermo Fisher Scientific, Waltham, MA), water control (“N”), RNA used from healthy male control subject (“C”). This demonstrates the ability to detect the presence of CHM RNA when it is expected to be present by creating and amplifying cDNA.