Figure 5. Immunofluorescent staining for expression of Smad7 and Hes1 on the ocular surface epithelium. A: Representative images from three independent experiments showing immunofluorescent staining of Hes1 and Smad7 in mouse corneal and limbal epithelia. Nuclei were visualized with Hoechst 33,258 staining (blue). Original magnification = 1,000X. B: Representative western blots from two independent experiments showing Smad7 and limbal epithelial stem cell (LSC) marker protein Hes1 and ∆Np63α expression by rat corneal and limbal epithelia.