Figure 2. Sodium iodate (SI) induces promoter activity and mRNA upregulation of calcium-independent phospholipase A2, group VIA. A: Luciferase activity in ARPE-19 cell cultures is increased in response to 1 mM SI exposure for 24 h. * indicates p<0.05 ±
SD (n=3). B: iPLA2-VIA and cPLA2-IVA mRNA expression in nonconfluent and confluent ARPE-19 cells after exposure to 1 mM SI for 24 h. A threefold induction
of both iPLA2-VIA and cPLA2-IVA mRNA was found in nonconfluent ARPE-19 cells. A tendency for downregulation of iPLA2-VIA was found when confluent ARPE-19 cells were exposed to SI (p = 0.08 ± SEM; n=3). However, no change was found in iPLA2-VIA or cPLA2-IVA expression when confluent cells were exposed to SI. ** indicates p<0.01± SEM (n=3) when mRNA levels from nonconfluent
ARPE-19 cells are compared with mRNA levels from SI-treated nonconfluent ARPE-19 cells.