Figure 4. Efficient suppression of choroidal neovascularization in vivo. One week after subretinal injection of adeno-associated virus type 8 vector/vascular endothelial growth factor (VEGF) AAV2/8/SmVEGF-2
(n = 12) or AAV2/8/GFP (n = 14), mice were subjected to laser injury. After an additional 2 weeks the eyes were examined,
and choroidal neovascularization (CNV) was analyzed using fluorescein angiography. CNV lesions in eyes injected with AAV2/8/SmVEGF-2
(D and E) were smaller than those in eyes injected with AAV2/8/green fluorescent protein (GFP; A and B). C: The magnification of square in the (B) showed the expression in RPE cells. Arrowheads: CNV lesions. Scale bar = 50 µm. A, B, D, and E, 150 µm; C.