Figure 3. Histological analysis of green fluorescent protein expression. Expression mediated by the adeno-associated virus type 8 vector/green fluorescent protein (AAV2/8/GFP) vector was strongly detected in the photoreceptor cells and RPE cells after subretinal injection (A and B). Retinal flat mount preparations also showed widespread GFP expression in RPE cells (C and D). Scale bar = 500 µm; A and C, 100 µm; B and D.