Figure 2. Transfection efficiency and siRNA-mediated vascular endothelial growth factor (VEGF) knockdown in 3T3 cells. A: Green fluorescent protein (GFP)-positive cells were measured using fluorescence –activated cell sorting (FACS) analysis. The transfection efficiency was nearly 65%. B: Efficacy screening of three siRNAs in 3T3 cells using semiquantitative RT–PCR. Note that the VEGF/GAPDH ratio was significantly smaller in cells transfected with the indicated SmVEGFs than the control vector. (pSmVEGF-1, pSmVEGF-2, pSmVEGF-3:0.77, 0.55, 0.55).