Figure 3. Retinal integrity of inducible RPE-specific Cre mice 12 months after intravitreal Dox injection (4 µg in 1 µl). A: Representative image of hematoxylin and eosin-stained retinal sections. Ctrl (control): age-matched wild-type littermate without Dox injection. Scale bar = 100 µm. B: Statistical analysis with the Student t test for photoreceptor outer nuclear layer (ONL) thickness. ONH: Optic nerve head. Error bar: standard deviation (SD); n = 6. No statistical significance was identified at any data point. Intravitreal Dox injection did not cause changes in retinal morphology in inducible RPE-specific Cre mice 12 months after induction.