Table 2 of
Pagoulatos, Mol Vis 2014; 20:441-457. Table 2 of
Pagoulatos, Mol Vis 2014; 20:441-457.
Table 2. Primary antibodies used for Immunohistochemistry, Immunofluorescense and western blotting.
| Antibody | Type | Source | Dilution IHC/IF | WB | Protein blocking | Incubation conditions |
|---|---|---|---|---|---|---|
| HIF-1α clone H1αlpha67 | Monoclonal mouse | Novus Biologicals | 1:25 | 1:500 | TBS/BSA 3% | ON 4 °C |
| pVHL clone (52A11) | Monoclonal mouse | Acris Antibodies | 1:100 | 1:500 | TBS/BSA 5% | ON 4 °C |
| Hsp90 | Monoclonal rabbit | Cell Signaling | 1:250 | 1:1,000 | TBS/BSA 10% | ON 4 °C |
| Hsp70 | Polyclonal rabbit | Cell Signaling | 1:100 | 1:500 | TBS/BSA 7% | ON 4 °C |
| Hsp27 Phospho-Hsp27 (Ser 82) β-tubulin | Monoclonal mouse Polyclonal rabbit Monoclonal mouse | Biogenex Cell Signaling Sigma | 1:80 1:50 | 1:1,000 1:1,000 1:2,000 | TBS/BSA 2% TBS/BSA 5% 5% nonfat milk | ON 4 °C ON 4 °C ON 4 °C |