Figure 4. Protein alignment of 10 (LRP5) orthologs demonstrates conservation in the regions with mutations. The 10 orthologs are from the following 10 species: Homo sapiens (NP_002326.2), Pan troglodytes (XP_508605.3), Canis lupus familiaris (XP_003432463.1), Bos taurus (XP_002699451.1), Macaca mulata [M. musculus] (NP_032539.2), Rattus norvegicus (NP_001099791.2), Gallus gallus (NP_001012915.1), Danio rerio (NP_001170929.1), Drosophila melanogaster (NP_524737.2), and Anopheles gambiae (XP_320740.4). The regions with the four missense mutations are highly conserved. A422T stands for Ala422Thr, L540P stands for Leu540Pro, Q816P stands for Gln816Pro, and T852M stands for Thr852Met.