Appendix 3 of Caprara, Mol Vis 2014; 20:307-324.


Appendix 3. Localization of CRE activity in the retina of Ai6;α-Cre reporter mice.

(A) Retinal cryosection of Ai6;α-Cre mice (cut naso-temporal) at post-natal day (PND) 15. ZSGREEN fluorescence (green) indicates cells that underwent CRE mediated recombination. Inset (A1) shows a higher magnification of the transition zone between central and peripheral retina. Scale bars: 200 μm and 50 µm (inset). (B) Identification of CRE-expressing retinal cells by the co-localization of ZSGREEN and different cell markers in Ai6;α-Cre mice at PND 15. Shown are retinal cryosections presenting native ZSGREEN fluorescence after CRE-mediated recombination (green) and immunostainings for different retinal cell markers (red) as indicated. POU domain, class 4, transcription factor 1 (POU4F1 (BRN3A)) for retinal ganglion cells (RGCs); calbindin 1 (CALB1) for horizontal cells and a subset of amacrine cells; calbindin 2 (CALB2) for amacrine cells and a subset of ganglion cells; glutamate-ammonia ligase (GLUL (GS)) for Müller cells; glial fibrillary acidic protein (GFAP) for astrocytes and activated Müller cells; visual system homeobox 2 (VSX2 (CHX10)) for bipolar cells; protein kinase C alpha (PKCA) for rod bipolar cells; retinal pigment epithelium-specific 65 kDa protein (RPE65) for the retinal pigment epithelium. Scale bar: 100 μm. RPE: retinal pigment epithelium; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer; D: dorsal; V: ventral. To access the data, click or select the words “Appendix 3.”