Molecular Vision: Caprara, Mol Vis 2014; 20:307-324. Appendix 2. Assessment of cross-contamination by semiquantitative real-time PCR of retinal layers isolated by laser capture microdissection.

Appendix 2. Assessment of cross-contamination by semiquantitative real-time PCR of retinal layers isolated by laser capture microdissection.

(A) POU domain, class 4, transcription factor 1 (Pou4f1 (Brn3a)), a marker for the ganglion cell layer. (B) visual system homeobox 2 (Vsx2 (Chx10)), a marker for the inner nuclear layer. (C) Guanine nucleotide binding protein, alpha transducin 1 (Gnat1), a marker for the outer nuclear layer. Tissue was isolated from of EpoR^flox/flox;Opn-Cre, and EpoR^flox/flox control littermates. Values were normalized to actin beta (Actb) and expressed relatively to the value of EpoR^flox/flox in the ONL, which was set to 1. Shown are mean values ± SD of at least 2 different mice. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. To access the data, click or select the words “Appendix 2.”