Figure 7. Melanization of A2E-treated ARPE-19 cells. A: Isolated lysosome preparations from the untreated control (left) and multiple 1 µM A2E-treated cells (right) show brown melanin pigment in the A2E-treated cells and not in the control cells. B: Increased expression of tyrosinase-related protein 1 (TYRP1) but not premelanosome protein 17 (PMEL17) was detected in immunoblots of isolated lysosomal fractions from sub-cellular fractionation of control (C) and multiple 1 µM A2E-treated (A2E)-treated ARPE-19 cells by density gradient ultracentrifugation, probed with anti-TYRP1 and anti-PMEL17 primary antibodies. Results shown are representative of three separate experiments.