Acetonitrile extracts of lysosomal fractions and synthetic melanin were analyzed using MALDI-TOF mass spectrometry. A: Lysosomal fractions isolated from multiple 1 µM A2E-treated ARPE-19 cells; B: Synthetic melanin standard. M/z peaks of 441, 495, 523, 537, 551, 565 were common between ARPE-19 lysosomal extracts and synthetic melanin spectra (see inset box in panel B for expansion of synthetic melanin masses between m/z 405 and 602), but the lower m/z peaks of 304, 329 and 332 found in synthetic melanin were not. Results shown are representative of three separate experiments. To access the data, click or select the words “Appendix 3.”