Figure 5. Transcriptional regulation by PAX6 and PAX6(5a) on motif 4–1 luciferase reporters. A: Distribution of the nucleotides in Pax6-binding motif 4–1. The DNA sequences for gene synthesis are aligned with the corresponding
motifs. The binding sites and flanking sequences are in upper and lower case, respectively. Evaluation of PAX6 (black bars)
and PAX6(5a; gray bars) series with 4–1 motif luciferase reporters in cotransfected P19 cells. The sample size n=6 from two
independent triplicates. The error bars represent the standard deviation. The significant fold-changes are indicated by the
asterisks. The range of the p values are: *** <0.001, ** 0.001~0.01, * 0.01~0.05.