Table 1 of
Chen, Mol Vis 2014; 20:259-269. Table 1 of
Chen, Mol Vis 2014; 20:259-269.
Table 1. Specific set of primers and conditions of PCR
| Gene | Primmer sequences (5′-3′) | Annealing temp(°C) | PCR cycles | PCR product (bp) |
|---|---|---|---|---|
| VEGF | F: TGGTCCCAGGCTGCACCCAT | 58 | 35 | 184 |
| R: CGCATCGCATCAGGGGCACA | ||||
| ADAMTS-1 | F: CTCCGCCTGCACGCCTTTGA | 58 | 35 | 171 |
| R: ATCGCCATTCACGGTGCCGG | ||||
| ADM | F: ATGAAGCTGGTTCCGTCG | 58 | 35 | 350 |
| R: ATCCTAAAGAA AGTGGGGAGCACTTC | ||||
| TSP-1 | F: GGTCGGCCTGCACTGTCACC | 58 | 35 | 179 |
| R: GGGGAAGCTGCTGCACTGGG |