Figure 7. Adrenomedullin_22–52 inhibited the activation of PI3K by high glucose. After HRECs were treated with high glucose or high glucose plus different concentrations of adrenomedullin_22–52 for 24 h, the protein level of PI3K was determined by western blot assay. A: Representative results from three independent runs of western blot. B: Ratios of PI3K to GAPDH were determined by western blot. The quantitative data of the ratios were collected from three independent experiments. All values represent Mean±SD (n=3). * p<0.05 versus the 30 mM group. # p<0.05 versus the 5 mM group.