Figure 4. Adrenomedullin_22–52 inhibited high-glucose-induced tube formation. The tube formation was determined by Matrigel assay. A: After culture under appropriate glucose conditions for 24 h, HRECs (1.5×10⁴ cells/well) were added to 96-well culture plates and incubated for 8 h to allow the formation of capillary-like structures. A magnification of × 200 was used to image the cells. B: The quantitative data of the capillary-like structures were obtained from three independent experiments. All values represent Mean±SD (n=3). * p<0.05 versus the 30 mM group. # p<0.05 versus the 5 mM group.