Figure 9. Effect of the inhibitor of programmed necrosis, necrostatin-1 (Nec-1; 30 µM), on the DNA fragmentation rate in the cultured media of retinal pigment epithelial (RPE) cells. The cells were cultured for 6 and 24 h in the presence of myricetin (200 µM; A) and quercetin (200 µM; B), respectively. As negative control, inactive necrostatin-1 (Nec-1i; 30 µM) was tested. The vehicle control was made with dimethyl sulfoxide (DMSO; 0.2%). Data are means±standard error of the mean (SEM) of three to seven independent experiments using cells from different donors, and are expressed in percent of untreated control (100%). Significant difference vs. untreated control: *p<0.05. Significant difference vs. quercetin and myricetin control, respectively: ●p<0.05.