Figure 2. Effect of myricetin on the expression of the vascular endothelial growth factor gene. mRNA levels were determined by real-time RT–PCR after stimulation of the cells with myricetin for 2 and 6 h, respectively. A. Dose-dependency of the myricetin effect. The concentration of myricetin (in µM) is given in the bars. B. The stimulatory effect of myricetin (100 µM) on the gene expression of vascular endothelial growth factor (VEGF) is decreased in the presence of the inhibitor of programmed necrosis, necrostatin-1 (Nec-1; 30 µM), but not in the presence of inactive necrostatin-1 (Nec-1i; 30 µM). Vehicle controls were made with methanol (0.2%) and dimethyl sulfoxide (DMSO; 0.1%), respectively. Data are means±standard error of the mean (SEM) of three to six independent experiments performed in triplicate using cells from different donors. Significant difference vs. untreated control: *p<0.05. Significant difference vs. myricetin: ●p<0.05.