Figure 2. NOX4 expression in retina and retinal endothelial cells is increased at postnatal day18 (P18) in the rat 50/10 oxygen-induced retinopathy model (ROP model). Western blots of NOX4 expression in retinal lysates from room air (RA)-raised pups at P0, P14, and P18 (A; ANOVA ***p<0.001; ***p<0.001 versus P0 by one-way ANOVA) and in the retina from RA- and ROP model raised pups at P18 (B, representative gels) and P14 (C, densitometry); immunohistochemistry (IHC) of NOX4 and lectin colabeling in retinal sections at P18 (D) and greater in the ROP model (E; **p<0.01 versus standard deviation [SD], RA; n = 4–5, two-way ANOVA).