Figure 3. The effect of puerarin against IL-1β-induced apoptosis in TR-iBRB2 cells. A: Determination of apoptosis with the annexin V-propidium iodide PI dual-staining assay after treatment with interleukin (IL)-1β (10 ng/ml) in the absence or presence of the indicated concentrations of puerarin. B: The percentage distribution of apoptotic cells. All data are expressed as mean ± standard deviation (SD) of three experiments, and each experiment included triplicate repeats **p<0.01 versus control; ^##p<0.01 versus interleukin (IL)-1β alone.