Figure 4. Western blot analysis of Bcl-2 and pBcl-2 in HLE-B3 cells treated with SB216763. The experiment was repeated three times with independent cell populations, and the levels of pBcl-2 and Bcl-2 in the cytoplasmic and nuclear fractions were quantified using ImageJ analysis. The increase in the pBcl-2 levels in the nuclear fractions of SB216763-treated cells as compared to the control cells was not statistically significant (p>0.05). There was no change in the levels of Bcl-2 or pBcl-2 in the cytoplasmic fractions. There was no significant carryover of the cytoplasmic markers in the nuclear fractions. SB=SB216763.