Figure 11. Effect of an HIF-2α translation inhibitor on HIF-2α levels in HLE-B3 cells. A: The experiment was repeated three times using independent cell populations. The normalized lysates were analyzed for levels of HIF-2α using ImageJ analysis Effect of an HIF-2α translation inhibitor on HIF-2α levels in HLE-B3 cells. The experiment was repeated three times using independent cell populations. The normalized lysates were analyzed for levels of HIF-2α using ImageJ analysis. Treatment of the cells with an HIF-2α inhibitor resulted in significant suppression of the HIF-2α protein levels (p<0.05). B: Effect of an HIF-2α translation inhibitor on VEGF levels in HLE-B3 cells. Cell-free supernatants were collected in triplicate from three independent cell populations after 3, 8, and 24 h of incubation in atmospheric oxygen. The supernatants were analyzed for VEGF levels by ELISA. Inhibition of HIF-2α in atmospheric oxygen did not influence the levels of VEGF as compared to control cells (p>0.05). C: Effect of an HIF-2α translation inhibitor on Bcl-2/pBcl-2 levels in HLE-B3 cells. Inhibition of HIF-2α did not alter the levels of Bcl-2/pBcl-2 levels as compared to the control cells. The experiment was repeated three times using three independent cell populations, and there was no statistically significant difference in the Bcl-2/pBcl-2 levels (p>0.05).