Figure 1. Analysis of GSK-3β enzymatic activity. The experiment was repeated three times using independent cell populations, and the ratio between pGSK-β/GSK-3β and pGS/GS was quantified using ImageJ analysis. Treatment of HLE-B3 cells with SB216763 resulted in inhibition of phosphorylation of glycogen synthase as compared to the untreated controls in both the cytoplasmic and nuclear fractions. The asterisk (*) indicates a significant decrease of pGS (p<0.05). There was no significant change in the levels of GSK-3β and phosphoglycogen synthase kinase-3β (pGSK-3β) between the control and SB 216,763 treated cells in both the cytoplasmic and nuclear lysates. There was no significant carryover of the cytoplasmic markers in the nuclear fractions. SB=SB216763.