Figure 4. Graft survival after keratoplasty and s.c. Tregs. A: Graft survival after keratoplasty in 3-week-old recipients with subconjunctival Treg application. Application of Tregs led to 66.7% graft survival in 3-week-old recipients (n=6; *p<0.01), application of CD4+CD25- cells induced a graft survival of 25% and a median of POD11 (n=4), while without cells, grafts were rejected at POD9. Recipients of syngenic grafts did not reject until the end of the experiment on POD29 (n=8; *p<0.01). B: Graft survival after keratoplasty in 10-week-old recipients with subconjunctival application. In the control group (CD4+CD25-) the median of rejection was POD 12, in Treg group (CD4+CD25+) median was POD 16 and 37.5% of grafts survived POD35 (both n=6; *p<0.01) while in allogenic transplantations without addition of cells, rejection occurred on POD15 (n=4). In syngenic transplantations no rejection occurred until the end of the experiment on POD 35 (n=4).