Figure 4. RT–PCR assay of transcripts present in 28-day-old mouse lenses. Amplified cDNA products corresponding to PSD-95 exon 17–18–19
and CaMKIIα variable exon 15 are shown.A: cDNA product resolved with electrophoresis using PSD-95 exon-specific primers. The adjacent nucleotide histogram shows the
nucleotide sequence demonstrating the exon 18–19 splice junction and the corresponding amino acid sequence. B: cDNA product resolved with electrophoresis corresponding to the CaMKIIα transcripts amplified from the variable region.
The adjacent nucleotide histogram shows sequences corresponding to the upstream and downstream exon for exon 15.