Figure 1. Post-synaptic protein 95 (PSD-95) and polypyrimidine PTBP2 (nPTB) proteins are expressed in approximately 4-month-old rabbit and 28-day-old mouse lenses. A: Immunoblot detection of about 100 kDa PSD-95 in two individual rabbit lens samples. B: Detection of PSD-95 in two mouse lens samples of four lenses. C: Detection of about 50 kDa PTBP2 (nPTB) in rabbit lens. D: In situ detection of PTBP2 in rabbit and mouse lens. Photos show peripheral lens fibers at the lens border: I) indirect immunofluorescence detecting PTBP2 in rabbit lens, II) no primary antibody control in rabbit lens, III) anti-PTBP2 mouse lens, and IV) no primary control in mouse lens (1:200 original magnification; asterisk adjacent to lens epithelial cells beneath capsule at the anterior surface).