Figure 2. Changes in the RNA splicing of RP11–18I14.1 subsequent to Pnn-knockdown in HCET cells. A: RP11–18I14.1 contains six exons spanning 11 kb. RP18I14.1 from corneal epithelia cells contains exons 1–2, partial 4, partial 5, and 6. See Appendix 2. B: Induced knockdown of Pnn resulted in a change in the isoform pattern of RP18I14.1, with a decrease in the predominant exon 1, 2, and 5 isoform and the corresponding increases of isoforms containing one or two stretches of exon 4.