Figure 7. Multiple sequence alignments of βB2-crystallins. The protein sequences were aligned from the same region of the βB2-crystallins from 15 different species using MacVector. The numbering across the top is based on the human sequence. The light-blue boxes show 100% sequence identity in all species, the darker blue boxes show the consensus sequence, and the yellow boxes show mismatches. The amino acids that are altered in this family are boxed in thick black. Two of the amino acids that were altered in our congenital cataract family are completely conserved among the species studied, and one is highly conserved.