Figure 3. Effect of daily systemic valproic acid (VPA; P17–P28, late treatment) on neurotrophic factor and rod-specific gene expression
in the wild-type mouse retina. A daily dose of VPA (350 mg/kg) was administered by intraperitoneal injection starting at age
P17. Littermates received injections of PBS only. Gene expression, relative to PBS-injected littermates at age P28 was measured
using real-time PCR for the gene of interest (Gdnf, Bdnf, Cntf, Fgf2, Mef2c, Nrl, Crx, Nr2E3, Rho, Pde6b) and was normalized to Actb expression (mean±standard deviation; n = 3/group, t test, *p<0.05, **p<0.01, relative to PBS).