Appendix 1 of Mitton, Mol Vis 2014; 20:1527-1544.


Appendix 1. Systemic VPA treatment of wild-type mice from P17-P28 (later treatment) had no effect on retinal morphology.

To access the data, click or select the words “Appendix 1.” Examples of b-scans are shown from VPA-treated and PBS-treated littermates. Average ONL (outer nuclear layer) thickness was the same between both groups. Using InVivoVue Diver software, 24 different measurement locations were obtained from a 5×5 rectangular grid (1.4×1.4 mm, measurement was not made at one grid position centered on the optic disc.) The average ONL thicknesses were: 63±2 µm for PBS-treated littermates (n=3 mice) and 63±2 µm for VPA-treated littermates (n=3 mice). Vertical (red) and horizontal (green) scale bars represent millimeters.