Figure 6. Data show rMC-1 cells were grown in normal glucose (NG) or high glucose (HG) and transfected with an IRS-1 plasmid or a mutated IRS-1 plasmid and treated with SOCS3 (SOCS3) or SOCS3+mutant. Data shows that SOCS3 requires IRS-1^Ser307 to regulate IR^Tyr960. *p<0.05 versus NG non-treated; #p<0.05 versus HG non-treated; $p<0.05 versus HG SOCS3. n=5 for all groups. Data are mean±standard error of the mean (SEM).