Figure 1. Cell viability was determined by adenosine 5′-triphosphate (ATP) assay. Primary mouse retinal ganglion cells (RGCs) were exposed to H₂O₂ for up to 16 h. In the presence of maltol, the oxidative stress-induced cytotoxicity was significantly decreased in a dose-dependent manner. At concentrations of maltol higher than 1 mM, the cell viability became even greater compared to the untreated control. This ATP assay was conducted in quadruplicate and repeated at least three times from different cell harvests; cell viability data were expressed as mean ± standard error of the mean (SEM).