Figure 3. Toll-like receptors signaled via MyD88. Human conjunctival fibroblasts were exposed to 20 ng/ml of core and NS3 proteins for 6 h. Immunofluorescence staining was performed for MyD88 and nuclear factor-kappa B (NF-kB). A: At the 8 h time point, MyD88 fluorescent intensity increased in the core and NS3-treated cells compared to the untreated control. B: At the 24 h time point, the fluorescent signal for the core and NS3-treated cells was similar to that of the control cells. C–D: At the 8 and 24 h time points, NF-kB nuclear translocation was not observed for the core and NS3-treated cells. Data are representative of three independent experiments. Scale bar=50 μm.