Figure 4. Colocalization of RAGE with Brn3a and PARP in the IR-injured rat retina at the12 h post-ischemia-reperfusion period. A: The transcription factor BRN3a (green) is present in a subset of cells in the retinal ganglion layer (RGC) layer at the12 h post-ischemia-reperfusion period. B: eRAGE (red) is localized to cells in the RGC layer in the ischemia reperfusion (IR)-injured rat retina at 12 h post-IR. C: Double immunolabeling with the eRAGE antibody (red) and the ganglion cell marker, BRN3a (green), at the 12 h reperfusion time shows they are colocalized. D: Necrotic cell marker, poly ADP-ribose polymerase (PARP) (green), is localized to cells in the RGC layer in the IR-injured rat retina at 12 h post-IR. E: eRAGE (red) is localized to cells in the RGC layer in the IR-injured rat retina at the 12 h post-ischemia-reperfusion period. F: Double immunolabeling with the RAGE antibody (red) and PARP at 12 h post-IR shows they are colocalized. Scale bars=20 μm.