Figure 3. Dual RNAscope in situ hybridization (ISH) and GFAP immunohistochemistry (IHC). Representative images of dual RNAscope ISH (red) and GFAP IHC (B, C, E, F, H, I, Müller cells, green) using mRNA probes for VEGF (A, C), HIF-1α (D, F) and HIF-2α (G, I). Nuclei were labelled with 4’,6-diamidino-2-phenylindole (DAPI) to distinguish the nuclear layers of the retina. All images were obtained using a 40× objective. Labels: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL).