Figure 2. Dual RNAscope in situ hybridization (ISH) and GS-lectin immunohistochemistry (IHC). Representative images of dual RNAscope ISH (red) and GS-lectin IHC (B, C, E, F, H, I, endothelial cells, green) using mRNA probes for VEGF (A, C), HIF-1α (D, F) and HIF-2α (G, I). Nuclei were labelled with 4’,6-diamidino-2-phenylindole (DAPI) to distinguish the nuclear layers of the retina. All images were obtained using a 40× objective. Labels: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL).