Table 3 of
Smith, Mol Vis 2014; 20:1328-1356. Table 3 of
Smith, Mol Vis 2014; 20:1328-1356.
Table 3. Antibodies against acetylcholine receptors
| Antibody (antigen) | Species | Catalog No. | Supplier |
|---|---|---|---|
| α1, α3, α5 nAChR A synthetic peptide corresponding to the main immunogenic region on the extracellular surface of muscle α1 AChR subunit, as well as the α3 and α5 subunits of human neuronal AChRs. The antibody binds the α1 subunit weakly when the protein is denatured, but can only detect the α3 and α5 subunit in native forms. | rat | mAb 210 (1:200 IHC) | Gift of Dr Jon Lindstrom, University of Pennsylvania |
| rat | AB24719 (1:1000 Western) | Abcam, Cambridge MA | |
| α9 nAChR A synthetic peptide corresponding to the N-terminal region of the human nAChR α9 protein. | rabbit | AB49065 (1:100 IHC) (1:1000 Western) | Abcam, Cambridge MA |
| m1 mAChR A Glutathione S-transferase (GST) fusion protein corresponding to a part of the i3 intracellular loop of human m1 mAChR protein (227–353). | rabbit | AB5164 (1:100 IHC) (1:50 Western) | Chemicon, Temecula CA |
| m2 mAChR A GST fusion protein corresponding to a part of the i3 intracellular loop of the m2 receptor (225–359). | rat | mAB367 (1:100 IHC) (1:100 Western) | Chemicon, Temecula CA |
| Choline Acetyltransferase/ChAT Protein corresponding to the human placental enzyme. | goat | AB144P (1:200 IHC) | Chemicon, Temecula CA |